2017/08/31 by Grzegorz Sikora, Agnieszka Wyłomańska, Janusz Gajda +4
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · Neuroscience · Physics and Astronomy · #Biochemistry #Biology #Biophysics #Channel (broadcasting) #Chemistry #Computer science #Hippocampal formation #Ion channel #Kinetics #Lipid Membrane Structure and Behavior #Materials science #Nanoclusters #Nanopore and Nanochannel Transport Studies #Nanotechnology #Neuroscience #Particle (ecology) #Physics #Receptor #Receptor–ligand kinetics #Spectroscopy and Quantum Chemical Studies #Surface (topology) #Tracking (education) #physics.bio-ph
paper · pdf · doi:10.1103/physreve.96.062404
published as Phys. Rev. E 96, 062404 (2017)
arxiv created 2017/11/29 · openalex publication_date 2017/12/11 · arxiv updated 2017/12/20 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/06
Protein and lipid nanodomains are prevalent on the surface of mammalian cells. In particular, it has been recently recognized that ion channels assemble into surface nanoclusters in the soma of cultured neurons. However, the interactions of these molecules with surface nanodomains display a considerable degree of heterogeneity. Here, we investigate this heterogeneity and develop statistical tools based on the recurrence of individual trajectories to identify subpopulations within ion channels in the neuronal surface. We specifically study the dynamics of the K+ channel Kv1.4 and the Na+ channel Nav1.6 on the surface of cultured hippocampal neurons at the single-molecule level. We find that both these molecules are expressed in two different forms with distinct kinetics with regards to surface interactions, emphasizing the complex proteomic landscape of the neuronal surface. Further, the tools presented in this work provide new methods for the analysis of membrane nanodomains, transient confinement, and identification of populations within single-particle trajectories.