Detrimental interactions of neonicotinoid pesticide exposure and bumblebee immunity

2017/06/01 by Mitchell Andrew Czerwinski, Ben M. Sadd · 51 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Insect and Pesticide Research #Plant and animal studies #Insect and Arachnid Ecology and Behavior #Neonicotinoid #Imidacloprid #Bumblebee #Biology #Immunity #Pesticide #Immune system #Toxicology #Pollinator #Zoology #Ecology #Immunology #Pollination

paper · doi:10.1002/jez.2087

published in Journal of Experimental Zoology Part A Ecological and Integrative Physiology 327(5), 273-283 (Wiley)

openalex publication_date 2017/06/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/05/21

Abstract

Pesticides are well known to have a number of ecological effects. However, it is only now becoming understood that sublethal exposures may have effects on nontarget insects of conservation concern through interactions with immunity, thus increasing detrimental impacts in the presence of pathogens. Pesticides and pathogens are suggested to have played a role in recent declines of several wild bee pollinators. Compromised immunity from exposure to widely used neonicotinoids has been demonstrated in honeybees, but further research on interactions between neonicotinoids and immunity in other important bees is lacking. In this study, adult workers of the bumblebee Bombus impatiens received 6-day pulses of either low (0.7 ppb) or high (7 ppb) field realistic doses of the neonicotinoid imidacloprid prior to assaying immunity and survival following a nonpathogenic immune challenge. High-dose imidacloprid exposure reduces constitutive levels of phenoloxidase, an enzyme involved in melanization. Hemolymph antimicrobial activity initially increases in all groups following an immune challenge, but while heightened activity is maintained in unexposed and low imidacloprid dose groups, it is not maintained in the high exposure dose bees, even though exposure had ceased 6 days prior. Additionally, imidacloprid exposure followed by an immune challenge significantly decreased survival probability relative to control bees and those only immune challenged or imidacloprid exposed. A temporal lag for immune modulation and combinatorial effects on survival suggest that resource-based trade-offs may, in part, contribute to the detrimental interactions. These interactions could have health consequences for pollinators facing multiple stresses of sublethal neonicotinoid exposure and pathogens.

Citations

• Fitting Linear Mixed-Effects Models Using lme4
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Cited by

• An update of the Worldwide Integrated Assessment (WIA) on systemic insecticides. Part 2: impacts on organisms and ecosystems
• Neonicotinoid Pesticides Cause Mass Fatalities of Native Bumble Bees: A Case Study From Wilsonville, Oregon, United States
• Pesticide toxicity to bees [wikipedia]

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