1991/01/01 by W. Galbraith, M. Wagner, Jean Chao +6 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Cell Image Analysis Techniques #Single-cell and spatial transcriptomics #Cancer Cells and Metastasis #Flow cytometry #Cytometry #Fluorescence #Fluorophore #Fluorescence microscope #Microscopy #Microscope #Pathology #Fluorescence-lifetime imaging microscopy #Biology #Molecular biology #Medicine #Optics #Physics
paper · pdf · doi:10.1002/cyto.990120702
openalex publication_date 1991/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/24
A system is described for performing multicolor fluorescence image cytometry of cell preparations. After the setting up stage, the operation is automatic: the microscope fields are found and focused; then images are acquired for each fluorophore, corrected and analyzed, without any operator interaction. Human peripheral blood lymphocytes on microscope slides were used as a test system. In these experiments, three fluorescent antibodies were used to identify lymphocyte sub-populations, and a DNA content probe was used to identify all nucleated cells. The cell subset percentages determined by image cytometry were comparable to percentages obtained when cells from the same preparation were analyzed by flow cytometry. Multicolor fluorescence imaging cytometry can potentially be extended to the analysis of cells in smears, fine needle biopsies, imprints, and tissue sections.