2000/11/01 by Hélène Raux, Anne Flamand, Danielle Blondel · 5 citations
Immunology and Microbiology · Environmental Science · Biochemistry, Genetics and Molecular Biology · #Rabies epidemiology and control #Bacteriophages and microbial interactions #Bacillus and Francisella bacterial research #Dynein #Biology #Rabies virus #Microtubule #Cell biology #Complementary DNA #Immunoglobulin light chain #Molecular biology #Immunoprecipitation #Lyssavirus #Microtubule-associated protein #Virus #Mononegavirales #Virology #Rhabdoviridae #Genetics #Antibody #Gene #Paramyxoviridae
paper · doi:10.1128/jvi.74.21.10212-10216.2000
openalex publication_date 2000/11/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
The rabies virus P protein is involved in viral transcription and replication but its precise function is not clear. We investigated the role of P (CVS strain) by searching for cellular partners by using a two-hybrid screening of a PC12 cDNA library. We isolated a cDNA encoding a 10-kDa dynein light chain (LC8). LC8 is a component of cytoplasmic dynein involved in the minus end-directed movement of organelles along microtubules. We confirmed that this molecule interacts with P by coimmunoprecipitation in infected cells and in cells transfected with a plasmid encoding P protein. LC8 was also detected in virus particles. Series of deletions from the N- and C-terminal ends of P protein were used to map the LC8-binding domain to the central part of P (residues 138 to 172). These results are relevant to speculate that dynein may be involved in the axonal transport of rabies virus along microtubules through neuron cells.