2017/04/27 by Wenting Dai, W.T. Dai, Q.J. Wang +8 · 17 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Animal Genetics and Reproduction #Biochemistry #Biology #Endocrinology #Enzyme #Fatty acid synthesis #Gene #Glycolysis #Internal medicine #Involution (esoterism) #Lactation #Mammary gland #Milk Quality and Mastitis in Dairy Cows #Pentose phosphate pathway #Pregnancy #Protein Hydrolysis and Bioactive Peptides #Proteome #Western blot
paper · pdf · doi:10.3168/jds.2016-12366
published in Journal of Dairy Science 100(7), 5928-5935 (Elsevier BV)
openalex publication_date 2017/04/27 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
The objective of this study was to determine how bovine mammary protein profiles vary during lactation and the dry period. Three lactating and 3 nonlactating cows were selected for mammary gland tissue sampling. Compared with the mammary proteins in nonlactating cows, a total of 60 differentially expressed proteins (DEP, including 57 upregulated and 3 downregulated) were identified in lactating cows using 2-dimensional difference gel electrophoresis combined with mass spectrometry. These DEP included enzymes and proteins associated with various macromolecular metabolic processes, and appeared to promote the increased metabolic activity associated with milk synthesis and secretion. The increased DEP were primarily related to initiation, maintenance, and involution of lactation, and included proteins involved in glycolysis/gluconeogenesis, the tricarboxylic acid cycle, the pentose phosphate pathway, oxidative phosphorylation, aminoacyl-transfer RNA biosynthesis, and fatty acid biosynthesis. Identified DEP were further validated by real-time, reverse-transcription PCR and Western blot. Five new DEP associated with lactation were uniquely identified. This work provided some protein-associated insights to facilitate further investigation of the mechanisms underlying lactation in dairy cows.