1989/04/21 by Qui‐Lim Choo, George Kuo, Amy J. Weiner +3 · 2 citations
Agricultural and Biological Sciences · Medicine · #Animal Virus Infections Studies #Biology #Complementary DNA #DNA #Gene #Genetics #Genome #Hepatitis B Virus Studies #Molecular biology #Viral gastroenteritis research and epidemiology #Virology #clone (Java method)
paper · doi:10.1126/science.2523562
openalex publication_date 1989/04/21 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
A random-primed complementary DNA library was constructed from plasma containing the uncharacterized non-A, non-B hepatitis (NANBH) agent and screened with serum from a patient diagnosed with NANBH. A complementary DNA clone was isolated that was shown to encode an antigen associated specifically with NANBH infections. This clone is not derived from host DNA but from an RNA molecule present in NANBH infections that consists of at least 10,000 nucleotides and that is positive-stranded with respect to the encoded NANBH antigen. These data indicate that this clone is derived from the genome of the NANBH agent and are consistent with the agent being similar to the togaviridae or flaviviridae. This molecular approach should be of great value in the isolation and characterization of other unidentified infectious agents.