2000/07/06 by Iva Turyan, Tomokazu Matsue, Daniel Mandler · 3 citations
Chemistry · Chemical Engineering · Engineering · #Electrochemical Analysis and Applications #Analytical Chemistry and Sensors #Molecular Junctions and Nanostructures #Scanning electrochemical microscopy #Chemistry #Monolayer #Ultramicroelectrode #Self-assembled monolayer #Microelectrode #Nanotechnology #Glucose oxidase #Surface modification #Scanning electron microscope #Electrochemistry #Cystamine #Microscope #Characterization (materials science) #Biosensor #Cyclic voltammetry #Electrode #Materials science #Optics
paper · doi:10.1021/ac000046a
openalex publication_date 2000/07/06 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/16
A novel approach for micropatterning of surfaces with organic and biological microstructures using the scanning electrochemical microscope (SECM) is described. The approach is based on the introduction of the spatial resolution by local deposition of gold particles followed by monolayer formation and functionalization. Specifically, gold patterns were deposited locally on silicon wafers with the SECM as a result of the controlled anodic dissolution of a gold microelectrode. The gold patterns were further used as microsubstrates for assembling cystamine monolayers to which either fluoresceine isothiocyanate (FIT) or glucose oxidase (GOD) were covalently attached. Characterization of the organic monolayers, as well as the biological activity of the enzyme patterns, was carried out by fluorescence microscopy and the SECM, respectively.