2002/08/05 by Philippe Ellul · 2 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Botanical Research and Chemistry #Mediterranean and Iberian flora and fauna #Sesquiterpenes and Asteraceae Studies #Biology #Botany #Subgenus #Interspecific competition #Propidium iodide #Genome size #Nuclear DNA #Genus #Zoology #Mitochondrial DNA #Genome #Genetics
paper · pdf · doi:10.1093/aob/mcf194
openalex publication_date 2002/08/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
Flow cytometry, using propidium iodide and 4',6-diamidano-2-phenylindole staining, was used to estimate the nuclear DNA content (2C) and the proportion of A-T base pairs in 16 species of the Mediterranean genus Cistus. Genome sizes were shown to be constant within species, since no significant intraspecific variation in 2C DNA content was detected. At the genus level, up to about 1.5-fold differences in absolute DNA amounts were observed, ranging from 3.92 pg in C. crispus to 5.88 pg in C. monspeliensis. The (AT) : (GC) ratio was close to 1, and was similar for all species examined, ranging from 47.87% A-T content in C clusii, to 50.67% in C. populifolius. Pink-flowered species (subgenus Cistus) had lower DNA amounts than white-flowered species (subgenera Leucocistus and Halimioides). However, the distribution of DNA amounts in Cistus appeared to be continuous and did not permit a clear separation of infra-generic ranks in the genus.