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Thermophilic Archaeal Diversity and Methanogenesis from El Tatio Geyser Field, Chile

2016/03/31 by Megan F. Plenge, Annette S. Engel, Annette Summers Engel +2 · 3 citations
Chemistry · Engineering · Environmental Science · #Anaerobic Digestion and Biogas Production #Archaea #Bacteria #Biochemistry #Biology #Chemistry #Ecology #Environmental chemistry #Enzyme #Euryarchaeota #Hydrothermal circulation #Hydrothermal vent #Methane #Methane Hydrates and Related Phenomena #Methanogenesis #Methanomicrobiales #Methanosarcina #Microbial Community Ecology and Physiology #Thermophile

paper · doi:10.1080/01490451.2016.1168496

openalex publication_date 2016/03/31 · crossref created 2016/03/31 · crossref issued 2016/08/16 · crossref published 2016/08/16 · crossref published-online 2016/08/16 · crossref published-print 2017/03/16 · crossref deposited 2020/08/29 · openalex created_date 2025/10/10 · crossref indexed 2026/08/04 · openalex updated_date 2026/08/04

Abstract

The hydrothermal fluid chemistry at El Tatio Geyser Field (ETGF) in northern Chile suggests that biogenic CO2–CH4 cycling may play an important role in water chemistry, and relatively low sulfate (0.6–1 mM) and high molecular hydrogen (H2) concentrations (67–363 nM) suggest that methanogenic Archaea are present in ETGF microbial mats. In this study, δ13C analysis of dissolved inorganic carbon and methane was not indicative of biogenic methane production (δ13CCH4 values ranging from −15‰ to −5.3‰); however, methanogenic Archaea were successfully cultured from each of the hydrothermal sites sampled. Sanger sequencing using universal Archaea primers identified putative methanogenic orders with varying metabolic capabilities, including Methanobacteriales, Methanomicrobiales and Methanosarcinales.

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