2022/12/21 by Angelo Velle, Chiara Pesenti, Tommaso Grassi +11 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #MicroRNA in disease regulation #Cancer-related molecular mechanisms research #Circular RNAs in diseases #microRNA #Biology #Ovarian cancer #Transcriptome #Stage (stratigraphy) #Tumor progression #Gene expression profiling #Cancer #Cancer research #Oncology #Pathology #Gene #Gene expression #Genetics #Medicine
paper · pdf · doi:10.1002/ijc.34408
openalex publication_date 2022/12/21 · openalex created_date 2023/01/04 · openalex updated_date 2026/08/01
isomiRs, the sequence-variants of microRNA, are known to be tissue and cell type specific but their physiological role is largely unknown. In our study, we explored for the first time the expression of isomiRs across different Stage I epithelial ovarian cancer (EOC) histological subtypes, in order to shed new light on their biological role in tumor growth and progression. In a multicentric retrospective cohort of tumor biopsies (n = 215) we sequenced small RNAs finding 971 expressed miRNAs, 64% of which are isomiRs. Among them, 42 isomiRs showed a clear histotype specific pattern, confirming our previously identified miRNA markers (miR192/194 and miR30a-3p/5p for mucinous and clear cell subtypes, respectively) and uncovering new biomarkers for all the five subtypes. Using integrative models, we found that the 38% of these miRNA expression alterations is the result of copy number variations while the 17% of differential transcriptional activities. Our work represents the first attempt to characterize isomiRs expression in Stage I EOC within and across subtypes and to contextualize their alterations in the framework of the large genomic heterogeneity of this tumor.