2017/07/12 by Ayumi Yamagami, Chieko Saito, Miki Nakazawa +9 · 1 citation
Agricultural and Biological Sciences · Chemistry · #Plant Molecular Biology Research #Plant Stress Responses and Tolerance #Plant nutrient uptake and metabolism #Brassinosteroid #Degradation (telecommunications) #Elongation #Cell biology #Receptor #Chemistry #Cell #Computational biology #Biology #Arabidopsis #Biochemistry #Mutant #Computer science #Gene #Materials science #Telecommunications
paper · pdf · doi:10.1038/s41598-017-06016-2
openalex publication_date 2017/07/12 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Brassinosteroids (BRs), plant steroid hormones, play important roles in plant cell elongation and differentiation. To investigate the mechanisms of BR signaling, we previously used the BR biosynthesis inhibitor Brz as a chemical biology tool and identified the Brz-insensitive-long hypocotyl4 mutant (bil4). Although the BIL4 gene encodes a seven-transmembrane-domain protein that is evolutionarily conserved in plants and animals, the molecular function of BIL4 in BR signaling has not been elucidated. Here, we demonstrate that BIL4 is expressed in early elongating cells and regulates cell elongation in Arabidopsis. BIL4 also activates BR signaling and interacts with the BR receptor brassinosteroid insensitive 1 (BRI1) in endosomes. BIL4 deficiency increases the localization of BRI1 in the vacuoles. Our results demonstrate that BIL4 regulates cell elongation and BR signaling via the regulation of BRI1 localization.