2010/05/13 by Vincent Gloaguen, Véronique Brudieux, B. Closs +6 · 3 citations
Agricultural and Biological Sciences · Chemistry · #Polysaccharides and Plant Cell Walls #Seaweed-derived Bioactive Compounds #Polysaccharides Composition and Applications #Zostera marina #Polysaccharide #Cell wall #Chemistry #Botany #Biology #Stereochemistry #Biochemistry #Ecology
paper · doi:10.1021/np100092c
openalex publication_date 2010/05/13 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/22
Zosterin, an apiose-rich pectic polysaccharide, was extracted and purified from the sea grass Zostera marina. Structural studies conducted by gas chromatography and NMR spectroscopy on a purified zosterin fraction (AGU) revealed a typical apiogalacturonan structure comprising an alpha-1,4-d-galactopyranosyluronan backbone substituted by 1,2-linked apiofuranose oligosaccharides and single apiose residues. The average molecular mass of AGU was estimated to be about 4100 Da with a low polydispersity. AGU inhibited proliferation of A431 human epidermoid carcinoma cells with an approximate IC(50) value of 3 microg/mL (0.7 microM). In addition, AGU inhibited A431 cell migration and invasion. Preliminary experiments showed that inhibition of metalloproteases expression could play a role in these antimigration and anti-invasive properties. Autohydrolysis of AGU, which eliminated apiose and oligo-apiose substituents, led to a virtual disappearance of cytotoxic properties, thus suggesting a direct structure-function relationship with the apiose-rich hairy region of AGU.