2012/11/21 by Jyoti Parkash, Irène Cimino, Nicoletta Ferraris +5 · 1 citation
Medicine · Immunology and Microbiology · Neuroscience · #Hypothalamic control of reproductive hormones #Growth Hormone and Insulin-like Growth Factors #Reproductive System and Pregnancy #Integrin #Biology #Forebrain #Vomeronasal organ #Gonadotropin-releasing hormone #Internal medicine #Median eminence #Endocrinology #Conditional gene knockout #Cell biology #Extracellular matrix #Neuroscience #Hormone #Receptor #Central nervous system #Olfactory system #Gene #Medicine #Luteinizing hormone #Genetics
paper · pdf · doi:10.1523/jneurosci.3057-12.2012
openalex publication_date 2012/11/21 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Reproduction in mammals is dependent on the function of hypothalamic neurons whose axons project to the hypothalamic median eminence (ME) where they release gonadotropin-releasing hormone (GnRH) into a specialized capillary network for delivery to the anterior pituitary. These neurons originate prenatally in the nasal placode and migrate into the forebrain along the olfactory-vomeronasal nerves. The complex developmental events leading to the correct establishment of the GnRH system are tightly regulated by the specific spatiotemporal expression patterns of guidance cues and extracellular matrix molecules, the functions of which, in part, are mediated by their binding to β1-subunit-containing integrins. To determine the biological role of these cell-surface proteins in reproduction, Cre/LoxP technology was used to generate GnRH neuron-specific β1-integrin conditional KO (GnRH-Itgb1(-/-)) mice. Loss of β1-integrin signaling impaired migration of GnRH neurons, their axonal extension to the ME, timing of pubertal onset, and fertility in these mice. These results identify β1-integrin as a gene involved in normal development of the GnRH system and demonstrate a fundamental role for this protein in acquisition of normal reproductive competence in female mice.