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Multicolor Spectral Karyotyping of Human Chromosomes

1996/07/26 by Evelin Schröck, Stanislas du Manoir, Timothy Veldman +9 · 2 citations
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Genomic variations and chromosomal abnormalities #Chromosomal and Genetic Variations #Gene expression and cancer classification #Karyotype #Chromosome #Fluorescence in situ hybridization #Centromere #Human genome #Biology #Genetics #Hybridization probe #Cytogenetics #Genome #Computational biology #Molecular biology #DNA #Gene

paper · doi:10.1126/science.273.5274.494

openalex publication_date 1996/07/26 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23

Abstract

The simultaneous and unequivocal discernment of all human chromosomes in different colors would be of significant clinical and biologic importance. Whole-genome scanning by spectral karyotyping allowed instantaneous visualization of defined emission spectra for each human chromosome after fluorescence in situ hybridization. By means of computer separation (classification) of spectra, spectrally overlapping chromosome-specific DNA probes could be resolved, and all human chromosomes were simultaneously identified.

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