2014/01/01 by Giovanna Chessa, Valentina Chisu, Rosaura Porcu +1 · 1 citation
Immunology and Microbiology · Medicine · #Toxoplasma gondii Research Studies #Parasitic Infections and Diagnostics #Herpesvirus Infections and Treatments #Biology #Toxoplasma gondii #Toxoplasmosis #Polymerase chain reaction #Nested polymerase chain reaction #Restriction fragment length polymorphism #Genotyping #Genotype #Virology #Aborted Fetus #Molecular biology #Typing #Fetus #Genetics #Gene #Pregnancy #Antibody
paper · pdf · doi:10.1051/parasite/2014007
openalex publication_date 2014/01/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/05/19
During 2009-2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5'-SAG2, 3'-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.