Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980/10/01 by Frederick Sanger, F. Sanger, A.R. Coulson +6 · 14 citations
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Bacterial Genetics and Biotechnology #Bacteriophage #Bacteriophages and microbial interactions #Base sequence #Biology #Cloning vector #Complementary DNA #DNA #DNA sequencing #EcoRI #Escherichia coli #Gene #Genetics #Genomic library #In vitro recombination #Molecular biology #Molecular cloning #Nucleic acid sequence #Oligonucleotide #RNA and protein synthesis mechanisms #Recombinant DNA #Restriction enzyme #Restriction site #Sanger sequencing #Sequencing by ligation

paper · doi:10.1016/0022-2836(80)90196-5

crossref issued 1980/10/01 · crossref published 1980/10/01 · crossref published-print 1980/10/01 · openalex publication_date 1980/10/01 · crossref created 2004/10/24 · crossref deposited 2021/06/26 · openalex created_date 2025/10/10 · crossref indexed 2026/07/08 · openalex updated_date 2026/07/28

Citations

• A different genetic code in human mitochondria
• The use of thin acrylamide gels for DNA sequencing
• A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase

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• Frederick Sanger [wikipedia]