2002/08/07 by Andrea Martín‐Nalda, Norberto Gallino, Julio Gagliardi +7 · 1 citation
Medicine · Chemistry · #Contact Dermatitis and Allergies #Allergic Rhinitis and Sensitization #Dermatology and Skin Diseases #CCL17 #CXCR3 #Immunology #Chemokine #Allergic contact dermatitis #Chemokine receptor #CCR4 #Antigen #Fibronectin #Medicine #Chemistry #Inflammation #Allergy #Cell
paper · pdf · doi:10.1186/1471-5945-2-9
openalex publication_date 2002/08/07 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
BACKGROUND: Allergic Contact Dermatitis (ACD) is regarded as a T-cell-mediated delayed-type hypersensitivity reaction. We studied the kinetics of the expression of CS-1 fibronectin, thymus and activation-regulated chemokine (CCL17/ TARC) and different chemokine receptors (CR) in skin biopsies from individuals suffering from back problems, with the antigen responsible of their contact dermatitis and an irrelevant antigen. METHODS: Samples were taken at 2, 10, and 48 hours for histological and immunohistochemical studies using monoclonal antibodies against human CS-1 fibronectin, CCL17, CD3, CD68, CD49d, CXCR3, CCR5, and CCR3. RESULTS: At positive antigen stimulated sites there was an early expression of CS-1 fibronectin (2 hours), followed by CCL17 and a later accumulation of alplha4/beta1+ (CD49d), CD3+, CD68+, CXCR3+ and CCR5+ mononuclear cells. At 48 hours, approximately 59 % of infiltrating cells were CXCR3+, 42% CCR5+, and only 14 % CCR3+. CONCLUSIONS: These results showed for the first time a very early expression of CS-1 fibronectin which preceded production of CCL17 in blood endothelial cells (BCEs) from patients' skin with ACD. The role of these molecules in recruitment of monocytes and effector T cells in ACD is discussed.