2001/12/01 by Elisa Wurmbach, Tony Yuen, Barbara J. Ebersole +1 · 191 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Animal Genetics and Reproduction #Biology #Chemistry #Endocrinology #Gene #Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities #Genetics #Gonadotropin-releasing hormone #Hormone #Hormone receptor #Luteinizing hormone #Receptor #Reproductive Biology and Fertility #Thyrotropin-releasing hormone receptor
paper · pdf · doi:10.1074/jbc.m108716200
published in Journal of Biological Chemistry 276(50), 47195-47201 (Elsevier BV)
openalex publication_date 2001/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
An early gene cDNA microarray was developed to study genes that are regulated immediately following gonadotropin-releasing hormone (GnRH) receptor activation. 956 selected candidate genes were printed in triplicate, a t statistic-based regulation algorithm was used for data analysis, and the response to GnRH in a time course from 1 to 6 h was determined. Measurements were highly reproducible within arrays, between arrays, and between experiments. Accuracy and algorithm reliability were established by real-time polymerase chain reaction assays of 60 genes. Gene changes ranging from 1.3- to 31-fold on the microarray were confirmed by real-time polymerase chain reaction. Many of the genes were found to be highly regulated. The regulated genes identified were all elevated at 1 h of treatment and returned nearly or completely to baseline levels of expression by 3 h of treatment. This broad, robust, and transient transcriptional response to constant GnRH exposure includes modulators of signal transduction (e.g. Rgs2 and IkappaB), cytoskeletal proteins (e.g. gamma-actin), and transcription factors (e.g. c-Fos, Egr1, and LRG21). The interplay of the activators, repressors, and feedback inhibitors identified embodies a combinatorial code to direct the activity of specific downstream secondary genes.