Generation of medaka gene knockout models by target-selected mutagenesis

2006/12/08 by Yoshihito Taniguchi, Shunichi Takeda, Makoto Furutani‐Seiki +13 · 2 citations
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Animal Genetics and Reproduction #Chromosomal and Genetic Variations #Genomics and Chromatin Dynamics

paper · pdf · doi:10.1186/gb-2006-7-12-r116

openalex publication_date 2006/12/08 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23

Abstract

We have established a reverse genetics approach for the routine generation of medaka (Oryzias latipes) gene knockouts. A cryopreserved library of N-ethyl-N-nitrosourea (ENU) mutagenized fish was screened by high-throughput resequencing for induced point mutations. Nonsense and splice site mutations were retrieved for the Blm, Sirt1, Parkin and p53 genes and functional characterization of p53 mutants indicated a complete knockout of p53 function. The current cryopreserved resource is expected to contain knockouts for most medaka genes.

Cited by

• Essential Techniques for Introducing Medaka to a Zebrafish Laboratory—Towards the Combined Use of Medaka and Zebrafish for Further Genetic Dissection of the Function of the Vertebrate Genome
• Makoto Furutani-Seiki [wikipedia]