2003/08/20 by Gen Hua Yue, Dominic Ek Leong Ong, C C Wong +2 · 3 citations
Biochemistry, Genetics and Molecular Biology · #Identification and Quantification in Food #Genetic diversity and population structure #Genomics and Phylogenetic Studies
paper · doi:10.1046/j.1365-2109.2003.00949.x
openalex publication_date 2003/08/20 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
A sex-associated amplified fragment length polymorphism and a strain-specific random amplified polymorphic DNA marker were identified from Asian arowana (dragonfish; Scleropages formosus Müller & Schlegel) by screening pooled genomic DNA samples from three different strains as well as males and females respectively. Both markers were cloned, sequenced and successfully converted into sequence-tagged-site (STS) markers. The strain-specific STS marker could be applied to differentiate the Indonesian golden strain of Asian arowana from the green and blood-red strains before the stage when colours become identifiable. Individuals from the green strain could be sexed with an efficiency of 82.7% using the sex-associated STS marker. Thus, populations with preferred sex ratios can be formed without the need of rearing a large number of fish.