2009/03/10 by Pei‐Ji Zhao, Suo Gao, Liming Fan +5 · 1 citation
Pharmacology, Toxicology and Pharmaceutics · Biochemistry, Genetics and Molecular Biology · #Plant-based Medicinal Research #Ginseng Biological Effects and Applications #Bioactive Natural Diterpenoids Research
paper · doi:10.1021/np800657j
openalex publication_date 2009/03/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
To determine the biosynthesis pathway of the atisine-type diterpenoid alkaloids spiramines A/B and C/D, feeding experiments in in vitro cultured plantlets and enzymatic transformations in cell-free extracts were performed in combination with LCMS and tandem MS analyses. L-[2-(13)C,(15)N]Serine was used in the feeding experiments and enzymatic transformations, and the diterpene spiraminol was identified as a biosynthetic precursor of spiramine alkaloids. The LCMS and tandem MS spectra of the extracts from these experiments indicated that L-[2-(13)C,(15)N]serine was incorporated into spiramines A/B and C/D. The labeled reaction products show that l-serine is the one possible nitrogen source involved in the biosynthesis of atisine-type DAs.