2010/06/11 by Tae Kyoung Kim, James Eberwine · 1 citation
Biochemistry, Genetics and Molecular Biology · #RNA Interference and Gene Delivery #CRISPR and Genetic Engineering #Viral Infectious Diseases and Gene Expression in Insects
paper · pdf · doi:10.1007/s00216-010-3821-6
openalex publication_date 2010/06/11 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microscope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.