2022/08/01 by Alexey A. Dmitriev, A. A. Dmitriev, E. N. Pushkova +3
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Biology #Chromosomal and Genetic Variations #Computational biology #DNA sequencing #Gene #Genetics #Genome #Genomics and Phylogenetic Studies #Molecular breeding #Plant Genetic and Mutation Studies #Reference genome #Sanger sequencing #Whole genome sequencing
paper · doi:10.1134/s0026893322040045
crossref issued 2022/08/01 · crossref published 2022/08/01 · crossref published-print 2022/08/01 · openalex publication_date 2022/08/01 · crossref published-online 2022/08/08 · crossref created 2022/08/08 · openalex created_date 2025/10/10 · crossref deposited 2026/03/29 · crossref indexed 2026/07/29 · openalex updated_date 2026/07/30
The investigation of plant genomes is of great importance for basic research and practical breeding. In 1977, F. Sanger proposed a DNA sequencing method, which allowed the complete sequences of a number of genomes to be determined. Then high-throughput and cost-effective next-generation/second-generation sequencing methods, producing up to billions of short reads, made it possible to sequence genomes of a significant number of species and provided a breakthrough in plant genetic studies. Finally, third-generation sequencing technologies allowed the determination of single-molecule sequences up to a million nucleotides in length, which is key for high-quality genome assemblies. An important task is to obtain a pan-genome, which includes an entire set of nucleotide sequences presented in various genotypes of the same species. The sequencing of plant genomes made it possible to assess intraspecific polymorphism, identify key genes influencing the formation of significant features, and develop molecular markers of economically valuable traits and this has become the basis for the development of marker-assisted and genomic selection. This review provides information on the latest advances in sequencing technologies and the assembly of plant genomes, as well as the opportunities that they open up for basic and applied works.