2009/03/31 by Richard Trauger, Eva Corey, Damon A. Bell +5 · 2 citations
Medicine · Biochemistry, Genetics and Molecular Biology · #Prostate Cancer Treatment and Research #Estrogen and related hormone effects #Hormonal and reproductive studies
paper · pdf · doi:10.1038/sj.bjc.6604987
openalex publication_date 2009/03/31 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Androst-5-ene-3beta, 17beta-diol (AED) is an adrenal hormone that has been reported to sustain prostate cancer growth after androgen deprivation therapy (ADT). LNCaP cells express a mutated androgen receptor that confers the ability to respond not only to androgen but also to oestrogen and adrenal hormones such as AED, and thus provide a cell line useful for identifying compounds capable of inhibiting AED-stimulated cell growth. We sought to determine whether structurally related steroids could inhibit AED-stimulated LNCaP cell growth in vitro and tumour growth in vivo. We report here the identification of a novel androstane steroid, HE3235 (17alpha-ethynyl-5alpha-androstan-3alpha, 17beta-diol), with significant inhibitory activity for AED-stimulated LNCaP proliferation. This inhibitory activity is accompanied by an increase in the number of apoptotic cells. Animal studies have confirmed the cytoreductive activity of HE3235 on LNCaP tumours. The results suggest that this compound may be of clinical use in castration-resistant prostate cancer.