2018/02/28 by Flemming Hansen, Tove Atlung · 1 citation
Social Sciences · Biochemistry, Genetics and Molecular Biology · #Korean Peninsula Historical and Political Studies #Mitochondrial Function and Pathology #Nuclear Issues and Defense
paper · pdf · doi:10.3389/fmicb.2018.00319
openalex publication_date 2018/02/28 · openalex created_date 2018/03/29 · openalex updated_date 2026/08/01
More than fifty years have passed since the presentation of the Replicon Model which states that a positively acting initiator interacts with a specific site on a circular chromosome molecule to initiate DNA replication. Since then, the origin of chromosome replication, oriC, has been determined as a specific region that carries sequences required for binding of positively acting initiator proteins, DnaA-boxes and DnaA proteins, respectively. In this review we will give a historical overview of significant findings which have led to the very detailed knowledge we now possess about the initiation process in bacteria using Escherichia coli as the model organism, but emphasizing that virtually all bacteria have DnaA proteins that interacts with DnaA boxes to initiate chromosome replication. We will discuss the dnaA gene regulation, the special features of the dnaA gene expression, promoter strength, and translation efficiency, as well as, the DnaA protein, its concentration, its binding to DnaA-boxes, and its binding of ATP or ADP. Furthermore we will discuss the different models for regulation of initiation which have been proposed over the years, with particular emphasis on the Initiator Titration Model.