2020/03/09 by Antoine Cherix, Guillaume Donati, Blanca Lizarbe +4 · 1 citation
Medicine · Chemistry · Neuroscience · #Advanced MRI Techniques and Applications #Advanced NMR Techniques and Applications #Neuroscience and Neuropharmacology Research
paper · pdf · doi:10.1177/0271678x20910535
openalex publication_date 2020/03/09 · openalex created_date 2020/03/23 · openalex updated_date 2026/07/02
Hippocampus plays a critical role in linking brain energetics and behavior typically associated to stress exposure. In this study, we aimed to simultaneously assess excitatory and inhibitory neuronal metabolism in mouse hippocampus in vivo by applying 18 FDG-PET and indirect 13 C magnetic resonance spectroscopy ( 1 H-[ 13 C]-MRS) at 14.1 T upon infusion of uniformly 13 C-labeled glucose ([U- 13 C 6 ]Glc). Improving the spectral fitting by taking into account variable decoupling efficiencies of [U- 13 C 6 ]Glc and refining the compartmentalized model by including two γ-aminobutyric acid (GABA) pools permit us to evaluate the relative contributions of glutamatergic and GABAergic metabolism to total hippocampal neuroenergetics. We report that GABAergic activity accounts for ∼13% of total neurotransmission (V NT ) and ∼27% of total neuronal TCA cycle (V TCA ) in mouse hippocampus suggesting a higher V TCA /V NT ratio for inhibitory neurons compared to excitatory neurons. Finally, our results provide new strategies and tools for bringing forward the developments and applications of 13 C-MRS in specific brain regions of small animals.