2005/03/01 by Holm Zaehres, M. William Lensch, Laurence Dahéron +3 · 2 citations
Biochemistry, Genetics and Molecular Biology · #Biology #CRISPR and Genetic Engineering #Cell biology #Embryonic stem cell #Gene #Genetics #Pluripotent Stem Cells Research #RNA #RNA Interference and Gene Delivery #RNA interference #Stem cell
paper · pdf · doi:10.1634/stemcells.2004-0252
openalex publication_date 2005/03/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.