Wild-type p53 binds to the TATA-binding protein and represses transcription.

1992/12/15 by Edward Seto, Anny Usheva, Gerard P. Zambetti +5 · 2 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #RNA Research and Splicing #Cancer-related Molecular Pathways #RNA modifications and cancer

paper · doi:10.1073/pnas.89.24.12028

openalex publication_date 1992/12/15 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/24

Abstract

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Citations

• Identification of p53 as a Sequence-Specific DNA-Binding Protein
• Transcriptional Activation by Wild-Type but Not Transforming Mutants of the p53 Anti-Oncogene

Cited by

• Two cellular proteins that bind to wild-type but not mutant p53.
• P53 [wikipedia]