2016/09/01 by Corey Nislow, Lai Hong Wong, Amy Huei‐Yi Lee +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · #Fungal and yeast genetics research #RNA and protein synthesis mechanisms
paper · doi:10.1101/pdb.top080945
openalex publication_date 2016/09/01 · openalex created_date 2016/09/16 · openalex updated_date 2026/07/14
Constructed by a consortium of 16 laboratories, the Saccharomyces genome-wide deletion collections have, for the past decade, provided a powerful, rapid, and inexpensive approach for functional profiling of the yeast genome. Loss-of-function deletion mutants were systematically created using a polymerase chain reaction (PCR)-based gene deletion strategy to generate a start-to-stop codon replacement of each open reading frame by homologous recombination. Each strain carries two molecular barcodes that serve as unique strain identifiers, enabling their growth to be analyzed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays or through the use of next-generation sequencing technologies. Functional profiling of the deletion collections, using either strain-by-strain or parallel assays, provides an unbiased approach to systematically survey the yeast genome. The Saccharomyces yeast deletion collections have proved immensely powerful in contributing to the understanding of gene function, including functional relationships between genes and genetic pathways in response to diverse genetic and environmental perturbations.