2006/12/01 by Paula S. Salgado, Minni R. L. Koivunen, Eugene V. Makeyev +3 · 3 citations
Biochemistry, Genetics and Molecular Biology · #Photosynthetic Processes and Mechanisms #RNA and protein synthesis mechanisms #Bacterial Genetics and Biotechnology
paper · pdf · doi:10.1371/journal.pbio.0040434
openalex publication_date 2006/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
RNA silencing refers to a group of RNA-induced gene-silencing mechanisms that developed early in the eukaryotic lineage, probably for defence against pathogens and regulation of gene expression. In plants, protozoa, fungi, and nematodes, but apparently not insects and vertebrates, it involves a cell-encoded RNA-dependent RNA polymerase (cRdRP) that produces double-stranded RNA triggers from aberrant single-stranded RNA. We report the 2.3-A resolution crystal structure of QDE-1, a cRdRP from Neurospora crassa, and find that it forms a relatively compact dimeric molecule, each subunit of which comprises several domains with, at its core, a catalytic apparatus and protein fold strikingly similar to the catalytic core of the DNA-dependent RNA polymerases responsible for transcription. This evolutionary link between the two enzyme types suggests that aspects of RNA silencing in some organisms may recapitulate transcription/replication pathways functioning in the ancient RNA-based world.