Genetic applications of an inverse polymerase chain reaction.

1988/11/01 by Howard Ochman, Anne S. Gerber, Daniel L. Hartl · 4 citations
Biochemistry, Genetics and Molecular Biology · #Molecular Biology Techniques and Applications #RNA and protein synthesis mechanisms #CRISPR and Genetic Engineering

paper · pdf · doi:10.1093/genetics/120.3.621

openalex publication_date 1988/11/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.

Citations

• Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia
• Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

Cited by

• Universal Fast Walking for direct and versatile determination of flanking sequence
• A large-scale insertional mutagenesis screen in zebrafish
• Horizontal transfer of an adaptive chimeric photoreceptor from bryophytes to ferns
• Polymerase chain reaction [wikipedia]