Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection

1987/05/01 by Shirley Kwok, David H. Mack, Kary B. Mullis +5 · 2 citations
Immunology and Microbiology · Medicine · #HIV Research and Treatment #HIV/AIDS drug development and treatment #Cytomegalovirus and herpesvirus research

paper · pdf · doi:10.1128/jvi.61.5.1690-1694.1987

openalex publication_date 1987/05/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

Human immunodeficiency virus (HIV) has been associated with acquired immunodeficiency syndrome and related disorders. Assays to detect antibodies to HIV proteins have been developed and used to screen sera for the identification of individuals who have been exposed to the virus. Although these serological tests have significant sensitivity and specificity for detecting exposure to the virus, they do not provide direct identification of HIV. We report here the application of recently developed nucleic acid amplification and oligonucleotide-based detection procedures for the identification of HIV sequences in established infected cell lines and in cells cultured from infected individuals.

Citations

• Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

Cited by

• JC virus DNA is present in many human brain samples from patients without progressive multifocal leukoencephalopathy
• Polymerase chain reaction [wikipedia]