2011/11/08 by Martin Beck, Alexander Schmidt, Johan Malmstroem +7 · 6 citations
Chemistry · Biochemistry, Genetics and Molecular Biology · #Advanced Proteomics Techniques and Applications #Microbial Metabolic Engineering and Bioproduction #Genetics, Bioinformatics, and Biomedical Research
paper · pdf · doi:10.1038/msb.2011.82
openalex publication_date 2011/11/08 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least -10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution.