1973/10/01 by Paul D. Boyer, Richard L. Cross, William E. Momsen · 3 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Environmental Science · #Protein Structure and Dynamics #Mass Spectrometry Techniques and Applications #Physiological and biochemical adaptations
paper · doi:10.1073/pnas.70.10.2837
openalex publication_date 1973/10/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
The P(i) right arrow over left arrow HOH exchange reaction of oxidative phosphorylation is considerably less sensitive to uncouplers than the P(i) right arrow over left arrow ATP and ATP right arrow over left arrow HOH exchanges. The uncoupler-insensitive P(i) right arrow over left arrow HOH exchange is inhibited by oligomycin. These results and other considerations suggest that the relatively rapid and uncoupler-insensitive P(i) right arrow over left arrow HOH exchange results from a rapid, reversible hydrolysis of a tightly but noncovalently bound ATP at a catalytic site for oxidative phosphorylation, concomitant with interchange of medium and bound P(i). Such tightly bound ATP has been demonstrated in submitochondrial particles in the presence of uncouplers, P(i), and ADP, by rapid labeling from (32)P(i) under essentially steady-state phosphorylation conditions. These results lead to the working hypothesis that in oxidative phosphorylation energy from electron transport causes release of preformed ATP from the catalytic site. This release could logically involve energy-requiring protein conformational change.