2010/03/29 by Matthew L. Eaton, Kyriaki Galani, Sukhyun Kang +2 · 3 citations
Biochemistry, Genetics and Molecular Biology · #DNA Repair Mechanisms #Genomics and Chromatin Dynamics #RNA Research and Splicing
paper · pdf · doi:10.1101/gad.1913210
openalex publication_date 2010/03/29 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.