2012/01/30 by Yun Sik Choi, Weronika Patena, Andrew D. Leavitt +1 · 33 citations
Biochemistry, Genetics and Molecular Biology · #Biogenesis #Biology #Cancer-related molecular mechanisms research #Computational biology #Gene #Genetics #Polyadenylation #Population #RNA #RNA Research and Splicing #RNA modifications and cancer
paper · pdf · doi:10.1261/rna.029306.111
published in RNA 18(3), 394-401 (Cold Spring Harbor Laboratory Press)
openalex publication_date 2012/01/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/06
Nontemplated 3'-end oligouridylation of RNA occurs in many species, including humans. Unlike the familiar phenomenon of polyadenylation, nontemplated addition of uridines to RNA is poorly characterized in higher eukaryotes. Recent studies have reported nontemplated 3'-end oligouridylation of small RNAs and mRNAs. Oligouridylation is involved in many aspects of microRNA biology from biogenesis to turnover of the mature species, and it may also mark long mRNAs for degradation by promoting decapping of the protective 5'-cap structure. To determine the prevalence of oligouridylation in higher eukaryotes, we used next-generation sequencing technology to deeply examine the population of small RNAs in human cells. Our data revealed widespread nontemplated nucleotide addition to the 3' ends of many classes of RNA, with short stretches of uridine being the most frequently added nucleotide.