2014/12/08 by Carlos Macias-Romero, Marie Didier, Pascal Jourdain +6 · 6 citations
Biochemistry, Genetics and Molecular Biology · Chemical Engineering · #Advanced Fluorescence Microscopy Techniques #Analytical Chemistry and Sensors #Biological imaging #Computer science #Laser #Materials science #Optics #Physics #Second-harmonic generation #Spectroscopy Techniques in Biomedical and Chemical Research #Telecommunications #Throughput
paper · doi:10.1364/oe.22.031102
openalex publication_date 2014/12/08 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/06
Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging throughput of SHG microscopy by using a wide-field imaging scheme in combination with a medium-range repetition rate amplified near infrared femtosecond laser source and gated detection. The imaging throughput of this configuration is tested by measuring the optical image contrast for different image acquisition times of BaTiO₃ nanoparticles in two different wide-field setups and one commercial point-scanning configuration. We find that the second harmonic imaging throughput is improved by 2-3 orders of magnitude compared to point-scan imaging. Capitalizing on this result, we perform low fluence imaging of (parts of) living mammalian neurons in culture.