Construction of Biologically Functional Bacterial Plasmids In Vitro

1973/11/01 by Stanley N. Cohen, Annie Chang, Herbert W. Boyer +1 · 9 citations
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Bacterial Genetics and Biotechnology #DNA and Nucleic Acid Chemistry #Bacteriophages and microbial interactions

paper · doi:10.1073/pnas.70.11.3240

openalex publication_date 1973/11/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/03

Abstract

The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be biologically functional replicons that possess genetic properties and nucleotide base sequences from both of the parent DNA molecules. Functional plasmids can be obtained by reassociation of endonuclease-generated fragments of larger replicons, as well as by joining of plasmid DNA molecules of entirely different origins.

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