1986/05/01 by Soheil Mojiri, Sebastian Isbaner, Steffen Mühle +8 · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Cellular Mechanics and Interactions #Granular flow and fluidized beds #Iron and Steelmaking Processes #Micro and Nano Robotics #Mining and Gasification Technologies #Orbital Angular Momentum in Optics
paper · doi:10.1364/boe.419099
openalex publication_date 1986/05/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/01
High speed volumetric optical microscopy is an important tool for observing rapid processes in living cells or for real-time tracking of sub-cellular components. However, the 3D imaging capability often comes at the price of a high technical complexity of the imaging system and/or the requirement of demanding image analysis. Here, we propose a combination of conventional phase-contrast imaging with a customized multi-plane beam-splitter for enabling simultaneous acquisition of images in eight different focal planes. Our method is technically straightforward and does not require complex post-processing image analysis. We apply our multi-plane phase-contrast microscope to the real-time observation of the fast motion of reactivated <i>Chlamydomonas</i> axonemes with sub-µm spatial and 4 ms temporal resolution. Our system allows us to observe not only bending but also the three-dimensional torsional dynamics of these micro-swimmers.