2006/10/02 by Julie Hardouin, Magalie Duchateau, Raymonde Joubert‐Caron +1 · 2 citations
Chemistry · #Mass Spectrometry Techniques and Applications #Advanced Proteomics Techniques and Applications #Analytical Chemistry and Chromatography
paper · doi:10.1002/rcm.2725
openalex publication_date 2006/10/02 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
Nanoflow liquid chromatography/mass spectrometry (nanoLC/MS) has become a current tool in proteomics applications increasingly used in the search for new biomarkers. A new integrated microfluidic device (HPLC-Chip), coupled to ion trap mass spectrometry (ITMS), appears as an innovative and robust tool for improving the identifications commonly performed by nanoLC/MS/MS. We tested this device for the identification of proteins obtained from two-dimensional gel electrophoresis or chromatography. The chip allows the measurement of reproducible retention times that, in association with m/z ratios, was found useful for identifying peptide sequences without ambiguity. A sensitivity increase of a factor of at least 5-fold is obtained compared to the results obtained previously in our laboratory by conventional nanoLC/MS/MS on the same ion trap. We conclude that this recently available microfluidic device can be a valuable tool during biomarker discovery programs, particularly identifying low-abundance proteins.