Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

2013/01/01 by Venkatachalam Chokkalingam, Jurjen Tel, Florian Wimmers +5 · 2 citations
Engineering · #Innovative Microfluidic and Catalytic Techniques Innovation #Microfluidic and Capillary Electrophoresis Applications #Electrowetting and Microfluidic Technologies

paper · doi:10.1039/c3lc50945a

openalex publication_date 2013/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.

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• Single-cell barcoding and sequencing using droplet microfluidics
• Microfluidics [wikipedia]

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