2011/04/18 by Yoshitake Akiyama, Keisuke Morishima · 2 citations
Chemistry · Engineering · #3D Printing in Biomedical Research #Aggregate (composite) #Biochemistry #Biological system #Biology #Biophysics #Cell #Chemical physics #Chemistry #Cytotoxicity #Diamagnetism #Electrohydrodynamics and Fluid Dynamics #Gadolinium #In vitro #Magnetic field #Materials science #Microfluidic and Bio-sensing Technologies #Nanotechnology #Physics #Spheroid
paper · doi:10.1063/1.3581883
openalex publication_date 2011/04/18 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/09
This letter demonstrates formation of cell aggregates by a label-free three-dimensional (3D) cell assembly method, which is based on the magneto-Archimedes effect. Gadolinium complex was added to the culturing medium to enhance the diamagnetic property of the cells, by which label-free magnetic manipulation became possible. Cytotoxicity of the gadolinium complex was confirmed to be sufficiently low for exposure less than one day. With this method, the cells aggregated into an egg-shape in about 20 min and became a spheroid in one day. These results suggest that this method is a promising approach to construct arbitrarily-shaped 3D tissues.