2002/03/14 by Milan N. Stojanović, Tiffany Elizabeth Mitchell, Darko Stefanović · 3 citations
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced biosensing and bioanalysis techniques #Molecular Junctions and Nanostructures #DNA and Nucleic Acid Chemistry
paper · doi:10.1021/ja016756v
openalex publication_date 2002/03/14 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. Importantly, as the gates have oligonucleotides as both inputs and output, they open the possibility of communication between various computation elements in solution. The operation of these gates is conveniently connected to a fluorescent readout.