The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences

1993/01/01 by Bengt Bjellqvist, Graham J. Hughes, Christian Pasquali +5 · 5 citations
Chemistry · Biochemistry, Genetics and Molecular Biology · #Advanced Proteomics Techniques and Applications #RNA and protein synthesis mechanisms #Glycosylation and Glycoproteins Research

paper · doi:10.1002/elps.11501401163

openalex publication_date 1993/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/15

Abstract

The focusing positions in narrow range immobilized pH gradients of 29 polypeptides of known amino acid sequence were determined under denaturing conditions. The isoelectric points of the proteins calculated from their amino acid sequences matched with good accuracy the experimentally determined pI values. We show the advantages of being able to predict the position of a protein of known structure within a two-dimensional gel.

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