2014/04/10 by A. Y. Lee, Robert P. St.Onge, Michael Proctor +36 · 1 citation
Chemistry · Biochemistry, Genetics and Molecular Biology · #Various Chemistry Research Topics #Genetics, Bioinformatics, and Biomedical Research
paper · doi:10.1126/science.1250217
openalex publication_date 2014/04/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Genome-wide characterization of the in vivo cellular response to perturbation is fundamental to understanding how cells survive stress. Identifying the proteins and pathways perturbed by small molecules affects biology and medicine by revealing the mechanisms of drug action. We used a yeast chemogenomics platform that quantifies the requirement for each gene for resistance to a compound in vivo to profile 3250 small molecules in a systematic and unbiased manner. We identified 317 compounds that specifically perturb the function of 121 genes and characterized the mechanism of specific compounds. Global analysis revealed that the cellular response to small molecules is limited and described by a network of 45 major chemogenomic signatures. Our results provide a resource for the discovery of functional interactions among genes, chemicals, and biological processes.