2006/09/29 by Arnaud Amzallag, Cédric Vaillant, Mathews Jacob +6 · 2 citations
Biochemistry, Genetics and Molecular Biology · #RNA and protein synthesis mechanisms #Genomics and Chromatin Dynamics #Bacterial Genetics and Biotechnology
paper · pdf · doi:10.1093/nar/gkl675
openalex publication_date 2006/09/29 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.