2017/12/08 by Jakub Wudarski, Daniil Simanov, Kirill Ustyantsev +17 · 1 citation
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Planarian Biology and Electrostimulation #Developmental Biology and Gene Regulation #Marine Ecology and Invasive Species
paper · pdf · doi:10.1038/s41467-017-02214-8
openalex publication_date 2017/12/08 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated the M. lignano genome, including genome-wide mapping of transcription start regions, and show its utility by generating multiple stable transgenic lines expressing fluorescent proteins under several tissue-specific promoters. The reported transgenesis method and annotated genome sequence will permit sophisticated genetic studies on stem cells and regeneration using M. lignano as a model organism.