Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes

2007/06/01 by Saligrama Deepak, Kameswara Rao Kottapalli, Randeep Rakwal +5 · 1 citation
Biochemistry, Genetics and Molecular Biology · #Molecular Biology Techniques and Applications #CRISPR and Genetic Engineering #Identification and Quantification in Food

paper · doi:10.2174/138920207781386960

openalex publication_date 2007/06/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/31

Abstract

Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21st century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant. Keywords: Real-time PCR, applications, disease, microorganisms, pathogen, detection, quantification, plant-microbe interaction

Citations

• Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
• The Obligate Human Pathogen, Neisseria gonorrhoeae, Is Polyploid
• Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis

Cited by

• Genetic engineering [wikipedia]

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