1974/05/01 by John F. Morrow, Stanley N. Cohen, Annie Chang +3 · 5 citations
Biochemistry, Genetics and Molecular Biology · #Bacterial Genetics and Biotechnology #DNA Repair Mechanisms #CRISPR and Genetic Engineering
paper · pdf · doi:10.1073/pnas.71.5.1743
openalex publication_date 1974/05/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/07
Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.