DNA sequencing with chain-terminating inhibitors

1977/12/01 by Frederick Sanger, S. Nicklen, Alan Coulson · 164 citations
Environmental Science · Biochemistry, Genetics and Molecular Biology · #Bacteriophages and microbial interactions #RNA and protein synthesis mechanisms #Bacterial Genetics and Biotechnology

paper · doi:10.1073/pnas.74.12.5463

openalex publication_date 1977/12/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/03

Abstract

A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.

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